Device

Part:BBa_I766226:Design

Designed by: Nili Sommovilla   Group: iGEM07_UCSF   (2007-10-22)

p110alpha-EE under weak constitutive promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2349
    Illegal XbaI site found at 93
    Illegal XbaI site found at 2353
    Illegal PstI site found at 3235
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2349
    Illegal PstI site found at 3235
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2349
    Illegal BglII site found at 1685
    Illegal BamHI site found at 4037
    Illegal XhoI site found at 611
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2349
    Illegal XbaI site found at 93
    Illegal XbaI site found at 2353
    Illegal PstI site found at 3235
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2349
    Illegal XbaI site found at 93
    Illegal XbaI site found at 2353
    Illegal PstI site found at 3235
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2104


Design Notes

See the UCSF iGEM wiki for cloning approach and design.


Source

This is a composite device. See individual parts for source information

References